Journal
JOURNAL OF INVERTEBRATE PATHOLOGY
Volume 114, Issue 2, Pages 144-150Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2013.07.005
Keywords
Pathogencity; Bacterial infection; Bivalve disease; Lesion; Low temperature
Categories
Funding
- Knowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-EW-Q213, KZCX2-EW-QN201]
- NSFC [31001129]
- earmarked fund for Modern Agro-industry Technology Research System
Ask authors/readers for more resources
A fatal disease with apparent lesions on the adductor muscles of Yesso scallop Patinopecten yessoensis has occurred for three consecutive years from 2009 to 2011 in the northeastern China. This disease has been suspected to be closely associated with bacterial infections. In the present study, 96 bacteria were isolated from the lesions of moribund scallops. They were grouped into Vibrio (88 strains), Moritella (5 strains), Pseudomonas (2 strains) and Shewanella (1 strain) by 16S rDNA sequencing. In the Vibrio group, Vibrio splendidus was the predominant species, consisting of 43 strains. A strain of V. splendidus, named JZ6, was identified as a potential pathogen because it possessed two important virulence factors, Vsm and OmpU. Stain JZ6 was cold-adapted, and could surviveand exhibit haemolytic activity from 4 degrees C to 32 degrees C, with the highest activity observed at 10 degrees C. The pathogenicity of JZ6 was further confirmed by injection and immersion challenges to scallops at 10 degrees C. The cumulative mortality rates in the two, experiments were 80% and 45%, respectively, which were significantly higher (P < 0.05) than those of control and blank groups. The disorganisation of muscle fibres in challenged scallops was observed to be histopathologically identical to that in moribund Yesso scallop collected from the same farm. The results indicate that V. splendidus is the predominantly culturable bacteria from the lesions of diseased Yesso scallop, and JZ6 probably is the pathogenic agent to Yesso scallop in this low temperature environment. (C) 2013 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available