Journal
JOURNAL OF INVERTEBRATE PATHOLOGY
Volume 105, Issue 2, Pages 200-203Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2010.06.012
Keywords
Acute bee paralysis virus; Kashmir bee virus; Deformed wing virus; Multiplex RT-PCR; RNA-dependent RNA polymerase; Bumblebees
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Funding
- Science and Technology in Flanders (IWT, Brussels, Belgium) [OZM/080317]
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Bumblebees are commercially reared and transported worldwide mainly for pollination of greenhouse tomatoes. Three honeybee viruses have been reported in bumblebees: Acute bee paralysis virus, Kashmir bee virus and Deformed wing virus. We developed a multiplex RT-PCR with primers designed on highly conserved regions of the RNA-dependent RNA polymerase in order to detect a maximum range of viral variants. Rearing facilities and governmental organizations can now thoroughly screen bumblebee colonies with a cost-effective technique with an integrated internal amplification control (IAC) implementable in laboratories that strive for International Organization for Standardization (ISO) certification. (C) 2010 Elsevier Inc. All rights reserved.
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