Journal
JOURNAL OF INVERTEBRATE PATHOLOGY
Volume 100, Issue 2, Pages 127-130Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2008.11.002
Keywords
Tripneustes gratilla; Lesions; Cloning; FISH; Sea urchin; Bacterial infection
Categories
Funding
- FRFC [2.4.583.05]
- CUD (Cooperation Universitaire au Developpement) of the French Community of Belgium
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The bacterial community associated with skin lesions of the sea Urchin Tripneustes gratilla was investigated using 16S ribosomal RNA gene cloning and fluorescent in situ hybridization (FISH). All clones were classified in the Alphaproteobacteria, Gammaproteobacteria and Cytophaga-Flexibacter-Bacteroides (CFB) bacteria. Most of the Alphaproteobacteria were related to the Roseobacter lineage and to bacteria implicated in marine diseases. The majority of the Gammaproteobacteria were identified as Vibrio while CFB represented only 9% of the total clones. FISH analyses showed that Alphaproteobacteria, CFB bacteria and Gammaproteobacteria accounted respectively for 43%, 38% and 19% of the DAPI Counts. The importance of the methods used is emphasized. (C) 2009 Published by Elsevier Inc.
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