Journal
JOURNAL OF INVERTEBRATE PATHOLOGY
Volume 101, Issue 2, Pages 157-160Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2009.03.004
Keywords
Ascosphaera aggregata; Ascosphaera apis; Chalkbrood; Bee diseases; Storage
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Funding
- Danish National Research Foundation
- Danish Council for Strategic Research
- The Velux Foundation
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Survival rates of Ascosphaera aggregata and Ascosphaera apis over the course of a year were tested using different storage treatments. For spores, the Storage methods tested were freeze-drying and ultra-low temperatures, and for hyphae, freeze-drying, agar slants, and two methods of ultra-low temperatures. Spores of A. aggregata and A. apis stored well at -80 degrees C and after freeze-drying. A. aggregata hyphae did not store Well under any of the methods tested while A. apis hyphae survived well using cryopreservation. Spores produced from cryopreserved A. apis hyphae were infective. Long-term storage of these two important fungal bee diseases is thus possible. (C) 2009 Elsevier Inc. All rights reserved.
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