Journal
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
Volume 37, Issue 2, Pages 503-510Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/147323000903700226
Keywords
CANCER STEM CELLS; C6 CELL LINE; TUMOUR SPHERES; HUMAN GLIOMA
Funding
- National Natural Science Foundation of China (NSFC) [30672163]
- Natural Science Foundation of Shandong Province [Y2007C108]
- Shandong Province Doctoral Foundation [2008BS03058]
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Various malignant cancers have been found to contain a sub-population of stem cell-like tumour cells, or cancer stem cells (CSCs), however, culture methods for CSCs and the size of the fraction of CSCs in C6, which is a commonly used glioma cell line, remain controversial. In this study, we demonstrated that the C6 cell line contains a fraction of tumour cells that can form tumour spheres in a simplified serum-free neural stem cell medium and express CD133 and nestin, which are widely-used markers for brain CSCs. Immunohistochemistry and immunofluorescence confirmed the existence of CSCs both in the C6 cell line and C6 xenografts. Flow cytometry demonstrated that 4.02% of cells in the C6 cell line and 4.21% in the C6 xenografts presented as CSCs. These results confirm the fraction of CSCs in the C6 cell line and provide a simple and effective method for isolation of CSCs to study the initiation and progression of human glioma and, possibly, other malignant tumours.
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