Journal
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
Volume 38, Issue 10, Pages 463-468Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/jir.2018.0020
Keywords
human bronchial epithelial cells; antigen uptake; calcitonin gene-related peptides; Th1/Th2differentiation
Funding
- Scientific Research Foundation of Hunan Provincial Science Technology Department [2014 FJ3009]
- Scientific Research Fund of Hunan Provincial Education Department [14C1140]
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In this study we tried to explore whether calcitonin gene-related peptide (CGRP) regulates the potential antigen uptaking ability of human bronchial epithelial cells (HBECs) and promoting the differentiation of Th1/Th2. We found that CGRP increased the uptake of fluorescein isothiocyanate labeled ovalbumin (FITC-OVA) by HBECs using fluorescence microscopy and flow cytometry analysis. MTT assay showed that T cells proliferated in a dose-dependent manner in the presence of OVA-pretreated HBECs and CGRP inhibited the proliferation of T cells. CGRP decreased secretion of IFN-gamma, while it had no influence on secretion of IL-4 by ELISA. Our data suggest that CGRP enhanced HBECs antigen uptake ability and inhibits HBECs induced T cells proliferation.
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