Journal
JOURNAL OF INSECT PHYSIOLOGY
Volume 58, Issue 10, Pages 1299-1306Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jinsphys.2012.07.002
Keywords
CAPA; NOS/cGMP; Aedes aegypti; Malpighian tubules; Anti-diuresis; Diuresis
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada
- Ontario Ministry of Research and Innovation
Ask authors/readers for more resources
This study reveals that AedesCAPA-PVK-1 (GPTVGLFAFPRV-NH2) inhibits basal and serotonin stimulated fluid secretion in the Malpighian tubules of larval Aedes aegypti at femtomolar concentrations. Conversely 10(-4) mol l(-1) of the peptide stimulated fluid secretion rates. The diuretic effects of 10(-4) mol l(-1) AedesCAPA-PVK-1 and antidiuretic effects of 10(-15) mol l(-1) AedesCAPA-PVK-1 were abolished by protein kinase A (PKA) and protein kinase G (PKG) inhibition, respectively. Similar to the peptide, 10(-3) mol l(-1) cGMP stimulated fluid secretion but doses in the micromolar to nanomolar range inhibited fluid secretion of the Malpighian tubules. Stimulatory effects of cGMP were abolished by PICA inhibition and inhibitory effects of cGMP were abolished by PKG inhibition. Furthermore, the nitric oxide synthase inhibitor L-NAME attenuated the inhibitory effects of AedesCAPA-PVK-1 but did not affect inhibition by cGMP. Based on the results we propose that AedesCAPA-PVK-1 inhibits fluid secretion rates of larval Malpighian tubules via the NOS/cGMP/PKG pathway and that high doses of the peptide lead to diuresis through the cGMP mediated activation of PKA. (C) 2012 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available