Journal
JOURNAL OF INSECT PHYSIOLOGY
Volume 58, Issue 2, Pages 220-227Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jinsphys.2011.10.011
Keywords
Carbaryl; Glutathione S-transferase; Locusta migratoria manilensis; RNA interference; Insecticides
Categories
Funding
- National Natural Science Foundation of China [30810103907]
- Regular Research Program [30870302, 31172161, 21007036]
- Public Welfare Fund for Agriculture [200903021]
- China Postdoctoral Science Foundation [201003656, 20090451359]
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Glutathione S-transferases (GSTs) play a major role in detoxification of xenobiotics and resistance to insecticides in insects. In the present study, a sigma-class GST gene (LmGSTs3) was identified from the locust, Locusta migratoria manilensis. Its full-length cDNA sequence is 828 bp containing an open reading frame (ORF) of 612 bp that encodes 204 amino acid residues. The predicted protein molecular mass and pI are 23.4 kDa and 7.62, respectively. Recombinant LmGSTs3 was heterologously expressed in Escherichia coli as a soluble fusion protein. Its optimal activity was observed at pH 8.0. Incubation for 30 min at temperatures below 40 C scarcely affected activity. The LmGSTs3 at pH values between 4.0 and 11.0 retained more than 80% of its original activity. Ethacrynic acid and cibacron blue were very effective inhibitors of LmGSTs3 with I-50-values 1.7 and 3.7 mu M, respectively. In response to heavy metal (CuSO4, CdCl2) exposure there was a concentration-dependent and time-dependent decrease in activity. The nymph mortalities after carbaryl treatment increased 38.7% after LmGSTs3 were silenced. These results suggest that LmGSTs3 may be involved in carbaryl detoxification in L migratoria manilensis. (C) 2011 Elsevier Ltd. All rights reserved.
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