Journal
JOURNAL OF INORGANIC BIOCHEMISTRY
Volume 135, Issue -, Pages 68-76Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2014.02.014
Keywords
Pyrazole derivative; Metal(II) complexes; Cancer cells; Cytotoxic effect; DNA interactions
Funding
- Scientific Exchange Program between Switzerland
- New Member States of the EU
- University of Zurich
- COST Action [CM1105]
- Medical University of Lodz [503/3-066-02/503-01]
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Three new copper(II) complexes containing two different pyrazole bound ligands (1, 2) have been synthesized and characterized by IR, LSI-MS (liquid secondary ion mass spectrometry) and elemental analysis. H-1 NMR spectra of the organic ligands have been recorded. We describe the influence of these complexes on particular cancer cell lines and DNA structure by MTT-assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], APA (acid phosphatase activity)-assay or CD-spectroscopy and agarose gel electrophoresis methods, together with their physico-chemical properties such as lipophilicity and stability in aqueous solution. The cytotoxic effect on HUVEC (endothelial cells) for the most active complex 4 has been also investigated. Moreover, the ability of these complexes to induce apoptosis in cancer cells has been assessed by using fluorescence microscopy. Our results indicate that dichloridobis(1-[amino(thioxo)methyl]-5-hydroxy-3-phenyl-1H-pyrazole-kappa N2)copper(II) is the most potent complex among the tested complexes. (C) 2014 Elsevier Inc. All rights reserved.
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