Journal
JOURNAL OF INORGANIC BIOCHEMISTRY
Volume 121, Issue -, Pages 19-27Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2012.12.011
Keywords
Ruthenium complexes; G-quadruplex DNA; Comparative study; Spectral properties; Molecular docking
Funding
- National Natural Science Foundation of China [20901060, 31170776, 81171646]
- Fundamental Research Funds for the Central Universities
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Two new polypyridine ligands and their corresponding ruthenium(II) complexes have been prepared and characterized. The interactions of both complexes with human telomere quadruplex DNA (both the antiparallel basket and the mixed-hybrid G-quadruplex) have been studied by circular dichroism (CD), CD melting, UV-visible (UV-Vis), fluorescent intercalator displacement (FID) assays and molecular docking studies. The results show that both complexes can stabilize G-quadruplexes DNA and two complexes show different binding affinity for different G-quadruplexes DNA. The 1:1 stoichiometry was confirmed in the buffered solutions by the UV-Vis spectrophotometer using Job's plot method and molecular docking studies. We have also investigated the interaction between the complexes and duplex DNA to gain some insight into the selectivity of the complexes for G-quadruplex structures. FID studies have shown that the complexes have a modest selectivity for G-quadruplex versus duplex DNA. (c) 2012 Elsevier Inc. All rights reserved.
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