Journal
JOURNAL OF INORGANIC BIOCHEMISTRY
Volume 104, Issue 7, Pages 718-725Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2010.03.004
Keywords
Phosvitin; Uranium(VI); Complexation; ATR FT-IR; Infrared spectroscopy
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The complexation of uranium(VI) to variant functional groups of the highly phosphorylated protein phosvitin in aqueous solution was investigated by attenuated total reflection Fourier transform infrared (AIR FT-IR) spectroscopy. For the verification of the affinity of the actinyl ions to carboxyl and phosphate groups of the amino acid side chains, samples with different phosphate to uranium(VI) (P/U) ratios were investigated under denaturing conditions as well as in aqueous medium. From a comparative study with other heavy metal ions, i.e. Ba2+ and Pb2+, a strong coordination of U(VI) to carboxyl and phosphoryl groups can be derived. Furthermore, with increasing P/U ratios, a preferential binding of U(VI) to phosphoryl groups is indicated by the spectra of the batch samples. These findings are confirmed by spectra of aqueous U(VI) phosvitin complexes reflecting an explicit coordination of the uranyl ions to phosphate groups at a high P/U ratio. Our study provides a deeper insight into the molecular interactions between actinyl ions and protein, and can be conferred to other basic biomolecules such as polysaccharides and nucleic acids. (C) 2010 Elsevier Inc. All rights reserved.
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