Synthesis, double stranded DNA-binding and photocleavage studies of a functionalized ruthenium(II) complex with 7,7′-methylenedioxyphenyldipyrido[3,2-a:2′,3′-c]-phenazine

A new Ru(II) complex [Ru(phen)(2)(mdpz)](2+) (phen = 1,10-phenanthroline, mdpz = 7,7'-methylenedioxyphenyl-dipyrido-[3,2-a:2',3'-c]phonazine) has been synthesized and characterized in detail by elemental analysis, mass spectrometry and H-1 NMR spectroscopy. The interaction of the complex with calf thymus DNA was investigated by spectroscopic and viscosity measurements. The results suggest that the complex binds to DNA via an intercalative mode and serves as a molecular light switch for DNA. Moreover, the complex has been found to promote the photocleavage of plasmid DNA pBR322 under irradiation at 365 nm. The mechanism studies reveal that singlet oxygen (O-1(2)) plays a significant role in the photocleavage. (C) 2008 Elsevier Inc. All rights reserved.