Journal
JOURNAL OF INNATE IMMUNITY
Volume 2, Issue 6, Pages 508-521Publisher
KARGER
DOI: 10.1159/000317665
Keywords
Neutrophil; Polymorphonuclear leukocytes; Innate immunity; Mucosal immunity; Fungal infections; Bacterial infections; Moraxella catarrhalis; Staphylococcus aureus; Haemophilus influenzae; Candida albicans
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Funding
- Swedish Foundation for Strategic Research
- Swedish Research Council [58X-11217-14-3]
- Torsten and Ragnar Soderberg Foundation
- Swedish Cancer Society
- Karolinska Institutet
- Magnus Bergwall's Foundation
- Ake Wiberg's Foundation
- David and Astrid Hagelen Foundation
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Neutrophil-derived antimicrobial peptides and proteins (AMPs) play an important role in the defense against microbes. Absence of defense is illustrated by neutropenic patients with frequent bacterial and fungal infections. However, the specificity of the antimicrobial effects has not been adequately described. We set out to determine the specific antimicrobial pattern of polypeptides in neutrophils (polymorphonuclear leukocytes, PMNs) against 4 potential human pathogens: Moraxella catarrhalis, Staphylococcus aureus, Haemophilus influenzae and Candida albicans. Protein extracts of human PMNs were separated using high-performance liquid chromatography and fractions were assayed for antimicrobial activity. Fractions displaying antimicrobial activity were separated on SDS-PAGE and characterized using MALDI-MS. Depletion experiments were utilized to determine the contribution of each AMP to the antimicrobial effect. Among the identified AMPs, alpha-defensins 1-3, azurocidin, LL-37, lysozyme, calprotectin and lactotransferrin were studied in detail. We found a divergent pattern of killing, that is, certain peptides and proteins exhibited selective activity against specific pathogens, while others displayed a broader antimicrobial activity. alpha-Defensins, LL-37 and calprotectin were active against all species, while lactotransferrin exclusively inhibited growth of S. aureus. Conversely, azurocidin was active against all species except S. aureus. Our observations may shed light on bacterial resistance to AMPs and on the elimination of specific bacterial communities on mucosal surfaces. Copyright (C) 2010 S. Karger AG, Basel
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