Journal
JOURNAL OF INFECTIOUS DISEASES
Volume 209, Issue 8, Pages 1288-1296Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiu013
Keywords
Leishmania braziliensis; reactive oxygen species; human monocytes; nitric oxide; amastigotes
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Funding
- National Institute of Allergy and Infectious Diseases, National Institutes of Health [UO1 AI88650]
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Leishmania braziliensis are intracellular parasites that cause unique clinical forms of cutaneous leishmaniasis. Previous studies with other leishmania species demonstrated that reactive oxygen species (ROS) control promastigotes, the infective stage of the parasite, but not the amastigote form that exists in the mammalian host. Here we show that ROS inhibits growth of L. braziliensis amastigotes in resting monocytes, and that classical monocytes are primarily responsible for this control. ROS, but not nitric oxide, also contributed to killing of L. braziliensis by IFN-gamma activated monocytes. Furthermore, by gene expression profiling of human lesions we found greater expression of genes associated with ROS, but not nitric oxide, compared to normal skin. This study shows that ROS are important for control of L. braziliensis both at the initial stages of infection, as well as at later time points, and highlights that monocyte subsets may play different roles during leishmaniasis.
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