Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 45, Issue 11, Pages 993-1002Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-018-2068-7
Keywords
Clostridium acetobutylicum; NAD(P)H; Redox; FdNR; Ferredoxin; TER
Categories
Funding
- Department of Energy [DE-SC0014462]
- Natural Science Foundation of Fujian Province of China [2016J01148, 2016J01147]
- Army Research Office [W911NF0910119]
- U.S. Department of Energy (DOE) [DE-SC0014462] Funding Source: U.S. Department of Energy (DOE)
Ask authors/readers for more resources
Clostridium acetobutylicum is a natural producer of butanol, butyrate, acetone and ethanol. The pattern of metabolites reflects the partitioning of redox equivalents between hydrogen and carbon metabolites. Here the exogenous genes of ferredoxin-NAD(P)(+) oxidoreductase (FdNR) and trans-enoyl-coenzyme reductase (TER) are introduced to three different Clostridium acetobutylicum strains to investigate the distribution of redox equivalents and butanol productivity. The FdNR improves NAD(P)H availability by capturing reducing power from ferredoxin. A butanol production of 9.01g/L (36.9% higher than the control), and the highest ratios of butanol/acetate (7.02) and C-4/C-2 (3.17) derived metabolites were obtained in the C acetobutylicum buk(-) strain expressing FdNR. While the TER functions as an NAD(P)H oxidase, butanol production was decreased in the C. acetobutylicum strains containing TER. The results illustrate that metabolic flux can be significantly changed and directed into butanol or butyrate due to enhancement of NAD(P)H availability by controlling electron flow through the ferredoxin node.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available