Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 41, Issue 5, Pages 811-821Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-014-1427-2
Keywords
Yeast biodiversity; 454 pyrosequencing; Grape; Wine; DGGE; PCR-ITS-RFLP; DNA fingerprints
Categories
Funding
- French state through the National Research Agency, as part of the Investments for the Future program [ANR-11-INBS-0001]
- Regional Council of Burgundy
Ask authors/readers for more resources
We compared pyrosequencing technology with the PCR-ITS-RFLP analysis of yeast isolates and denaturing gradient gel electrophoresis (DGGE). These methods gave divergent findings for the yeast population. DGGE was unsuitable for the quantification of biodiversity and its use for species detection was limited by the initial abundance of each species. The isolates identified by PCR-ITS-RFLP were not fully representative of the true population. For population dynamics, high-throughput sequencing technology yielded results differing in some respects from those obtained with other approaches. This study demonstrates that 454 pyrosequencing of amplicons is more relevant than other methods for studying the yeast community on grapes and during alcoholic fermentation. Indeed, this high-throughput sequencing method detected larger numbers of species on grapes and identified species present during alcoholic fermentation that were undetectable with the other techniques.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available