Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 41, Issue 11, Pages 1637-1645Publisher
OXFORD UNIV PRESS
DOI: 10.1007/s10295-014-1515-3
Keywords
Budding yeast; Inhibitor resistance; Vanillin tolerance; Oxidoreductase; Antioxidant
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Funding
- National Basic Research Program of China [2011CB707405]
- National High Technology Research and Development Program of China [2012AA022106]
- National Natural Science Foundation of China [30970091]
- State Key Laboratory of Motor Vehicle Biofuel Technology [2013004]
- Independent Innovation Foundation of Shandong University [IIFSDU 2010TS059]
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The phenolic compounds present in hydrolysates pose significant challenges for the sustainable lignocellulosic materials refining industry. Three Saccharomyces cerevisiae strains with high tolerance to lignocellulose hydrolysate were obtained through ethyl methanesulfonate mutation and adaptive evolution. Among them, strain EMV-8 exhibits specific tolerance to vanillin, a phenolic compound common in lignocellulose hydrolysate. The EMV-8 maintains a specific growth rate of 0.104 h(-1) in 2 g L-1 vanillin, whereas the reference strain cannot grow. Physiological studies revealed that the vanillin reduction rate of EMV-8 is 1.92-fold higher than its parent strain, and the Trolox equivalent antioxidant capacity of EMV-8 is 15 % higher than its parent strain. Transcriptional analysis results confirmed an up-regulated oxidoreductase activity and antioxidant activity in this strain. Our results suggest that enhancing the antioxidant capacity and oxidoreductase activity could be a strategy to engineer S. cerevisiae for improved vanillin tolerance.
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