Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 39, Issue 12, Pages 1869-1874Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-012-1197-7
Keywords
Corynebacterium glutamicum; Gluconate kinase; L-ornithine production; NADPH; Pentose phosphate pathway
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Funding
- Advanced R&D supporting business between industry and University
- Small and Medium Business Administration, Republic of Korea
- Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ0080992011]
- Sangji University
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With the purpose of generating a microbial strain for l-ornithine production in Corynebacterium glutamicum, genes involved in the central carbon metabolism were inactivated so as to modulate the intracellular level of NADPH, and to evaluate their effects on l-ornithine production in C. glutamicum. Upon inactivation of the 6-phosphoglucoisomerase gene (pgi) in a C. glutamicum strain, the concomitant increase in intracellular NADPH concentrations from 2.55 to 5.75 mmol g(-1) (dry cell weight) was accompanied by reduced growth rate and l-ornithine production, suggesting that l-ornithine production is not solely limited by NADPH availability. In contrast, inactivation of the gluconate kinase gene (gntK) led to a 51.8 % increase in intracellular NADPH concentration, which resulted in a 49.9 % increase in l-ornithine production. These results indicate that excess NADPH is not necessarily rate-limiting, but is required for increased l-ornithine production in C. glutamicum.
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