Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 39, Issue 4, Pages 521-527Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-011-1058-9
Keywords
Biotransformation; Co-transformation; Ginsenoside Rb-1; Ginsenoside Rg(1); Minor ginsenoside
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Funding
- National Natural Science Foundation of China [20862017]
- National Ginseng Products Quality Supervision Inspection Center
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Rb-1 and Rg(1) are the major ginsenosides in protopanaxadiol and protopanaxatriol. Their content in ginsenosides was 23.8 and 17.6%, respectively. A total of 22 isolates of beta-glucosidase producing microorganisms were isolated from the soil of a ginseng field using Esculin-R2A agar. Among these isolates, the strain GH21 showed the strongest activities to convert ginsenoside Rb-1 and Rg(1) to minor ginsenosides compound-K and F-1, respectively. Ginsenosides Rb-1 and Rg(1) bioconversion rates were 74.2 and 89.3%, respectively. Meanwhile, the results demonstrated that the ginsenoside Rg(1) could change the biotransformation pathway of ginsenoside Rb-1 by inhibiting the formation of the intermediate metabolite gypenoside-XVII. GH21 was identified as a Cladosporium cladosporioides species based on the internal transcribed spacers (ITS) ITS1-5.8S-ITS2 rRNA gene sequences constructed phylogenetic trees.
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