Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 39, Issue 3, Pages 401-407Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-011-1017-5
Keywords
Butanol; Clostridium beijerinckii; Inhibitor tolerance; Low-energy ion implantation
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Funding
- 973 program of China [2011CB707405]
- public benefit research foundation of CAF [201004001]
- State Key Laboratory of Materials-Oriented Chemical Engineering Foundation of Nanjing University of Technology [ZK201001]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Qinglan project of Jiangsu Province
- The six talent summit of Jiangsu Province [06-A-047]
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Clostridium beijerinckii mutant strain IB4, which has a high level of inhibitor tolerance, was screened by low-energy ion implantation and used for butanol fermentation from a non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid (SAHHC). Evaluation of toxicity showed C. beijerinckii IB4 had a higher level of tolerance than parent strain C. beijerinckii NCIMB 8052 for five out of six phenolic compounds tested (the exception was vanillin). Using glucose as carbon source, C. beijerinckii IB4 produced 9.1 g l(-1) of butanol with an acetone/butanol/ethanol (ABE) yield of 0.41 g g(-1). When non-detoxified SAHHC was used as carbon source, C. beijerinckii NCIMB 8052 grew well but ABE production was inhibited. By contrast, C. beijerinckii IB4 produced 9.5 g l(-1) of ABE with a yield of 0.34 g g(-1), including 2.2 g l(-1) acetone, 6.8 g l(-1) butanol, and 0.5 g l(-1) ethanol. The remarkable fermentation and inhibitor tolerance of C. beijerinckii IB4 appears promising for ABE production from lignocellulosic materials.
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