Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 39, Issue 1, Pages 93-98Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-011-1003-y
Keywords
Calcium alginate; Immobilization; Repeated batch cultivation; Thermostable alkaline protease; Bacillus halodurans JB 99
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Funding
- Council of Scientific and Industrial Research, New Delhi [37/1297/07 EMR-II]
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The thermoalkalophilic Bacillus halodurans JB 99 cells known for production of novel thermostable alkaline keratinolytic protease were immobilized in calcium alginate matrix. Batch and repeated batch cultivation using calcium alginate immobilized cells were studied for alkaline protease production in submerged fermentation. Immobilized cells with 2.5% alginate and 350 beads/flask of initial cell loading showed enhanced production of alkaline protease by 23.2% (5,275 +/- A 39.4 U/ml) as compared to free cells (4,280 +/- A 35.4 U/ml) after 24 h. In the semicontinuous mode of cultivation, immobilized cells under optimized conditions produced an appreciable level of alkaline protease in up to nine cycles and reached a maximal value of 5,975 U/ml after the seventh cycle. The enzyme produced from immobilized cells efficiently degraded chicken feathers in the presence of a reducing agent which can help the poultry industry in the management of keratin-rich waste and obtaining value-added products.
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