Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 39, Issue 4, Pages 623-628Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-011-1066-9
Keywords
Cordyceps sinensis; Liquid culture; Exopolysaccharide; Tween 80; Mycelial morphology
Categories
Funding
- Hong Kong Polytechnic University
- UGC of Hong Kong Government (GRF) [PolyU 5028/09P, PolyU 5036/10P]
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This study investigated the effects of surfactant additives and medium pH on mycelial morphology and exopolysaccharide (EPS) production in liquid culture of a valuable medicinal fungus Cordyceps sinensis Cs-HK1. In the medium containing 20 g l(-1) glucose and 6 g l(-1) peptone as the sole nitrogen source, the Cs-HK1 fungal mycelia formed smooth and spherical pellets about 1.8-mm mean diameter. The mycelial pellets became less uniform at pH (4.0-5.0) lower than the optimum (pH 6.0) or turned to filamentous form at higher pH (8-9). Surfactants added to the medium inhibited pellet formation, resulting in smaller and looser pellets. Tween 80 exhibited a remarkable promoting effect on EPS production, increasing the EPS yield more than twofold at 1.5% (w/v), which was most probably attributed to the stimulation of EPS biosynthesis and release from the fungal cells by Tween 80.
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