Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 37, Issue 7, Pages 681-687Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-010-0711-z
Keywords
1,3-Propanediol; Organic acid mixture; Repeated fed-batch culture; Klebsiella pneumoniae
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Funding
- Department of Science and Technology, Jiangsu Province, China [BG2006036]
- Shanghai Leading Academic Discipline Project [B505]
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In fed-batch culture of Klebsiella pneumoniae, 1,3-propanediol production was growth associated, while the by-products, including lactic acid and ethanol, increased sharply as the cells grew slowly. When the fed-batch culture was supplied with a mixture of organic acids including citrate, fumarate and succinate, cell growth and 1,3-propanediol production increased significantly, whereas the by-products, especially lactic acid and ethanol, decreased sharply. High concentrations of PDO and acetate inhibited cell growth and PDO production. To improve the PDO production, repeated fed-batch culture with addition of the organic acid mixture was performed in a 5-l reactor. The fed-batch culture was repeated five times, and the 1,3-propanediol yield and concentration reached above 0.61 mol mol(-1) and 66 g l(-1), respectively, in 20 h for each cycle. Furthermore, the PDO productivity reached above 3.30 g l(-1) h(-1) in each cycle, which was much higher than that of the original fed-batch culture.
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