Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 36, Issue 5, Pages 721-726Publisher
OXFORD UNIV PRESS
DOI: 10.1007/s10295-009-0542-y
Keywords
Biotransformation; Cladosporium fulvum (syn. Fulvia fulva); Ginsenoside Rb-1; Ginsenoside Rd
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Funding
- Program for New Century Excellent Talents in Universities [NCET-05-0321]
- Specialized Research Fund for the Doctoral Program of Higher Education [20070200004]
- National Natural Science Foundation of China [30570417]
- PCSIRT [IRT0519]
- Analysis and Testing Foundation of Northeast Normal University
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Fourteen phytopathogenic fungi were tested for their ability to transform the major ginsenosides to the active minor ginsenoside Rd. The transformation products were identified by TLC and HPLC, and their structures were assigned by NMR analysis. Cladosporium fulvum, a tomato pathogen, was found to transform major ginsenoside Rb-1 to Rd as the sole product. The following optimum conditions for transforming Rd by C. fulvum were determined: the time of substrate addition, 24 h; substrate concentration, 0.25 mg ml(-1); temperature, 37A degrees C; pH 5.0; and biotransformation period, 8 days. At these optimum conditions, the maximum yield was 86% (molar ratio). Further, a preparative scale transformation with C. fulvum was performed at a dose of 100 mg of Rb-1 by a yield of 80%. This fungus has potential to be applied on the preparation for Rd in pharmaceutical industry.
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