Journal
JOURNAL OF IMMUNOLOGY
Volume 193, Issue 10, Pages 5338-5344Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1401380
Keywords
-
Categories
Funding
- National Institute of Allergy and Infectious Diseases [AI049313, AR048632]
- Burroughs Wellcome Fund for Translational Research
- Nederlands Wetenschappelijk Onderzoek [Meervoud 836.08.001]
Ask authors/readers for more resources
During infection and autoimmune disease, activation and expansion of T cells take place. Consequently, the TCR repertoire contains information about ongoing and past diseases. Analysis and interpretation of the human TCR repertoire are hampered by its size and stochastic variation and by the diversity of Ags and Ag-presenting molecules encoded by the MHC, but are highly desirable and would greatly impact fundamental and clinical immunology. A subset of the TCR repertoire is formed by invariant T cells. Invariant T cells express interdonor-conserved TCRs and recognize a limited set of Ags, presented by nonpolymorphic Ag-presenting molecules. Discovery of the three known invariant T cell populations has been a tedious and slow process, identifying them one by one. Because conservation of the TCR alpha-chain of invariant T cells is much higher than the beta-chain, and because the TCR alpha-chain V gene segment TRAV1-2 is used by two of the three known invariant TCRs, we employed next-generation sequencing of TCR alpha-chains that contain the TRAV1-2 gene segment to identify 16 invariant TCRs shared among many blood donors. Frequency analysis of individual clones indicates these T cells are expanded in many donors, implying an important role in human immunity. This approach extends the number of known interdonor-conserved TCRs and suggests that many more exist and that these TCR patterns can be used to systematically evaluate human Ag exposure.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available