Journal
JOURNAL OF IMMUNOLOGY
Volume 192, Issue 6, Pages 2689-2698Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1302064
Keywords
-
Categories
Funding
- Molecular and Cell Biology Program from the Russian Academy of Science
- Russian Foundation for Basic Research [12-04-33139, 12-04-00229, 14-04-01247]
- European Regional Development Fund [CZ.1.05/1.1.00/02.0068]
Ask authors/readers for more resources
The decrease of TCR diversity with aging has never been studied by direct methods. In this study, we combined high-throughput Illumina sequencing with unique cDNA molecular identifier technology to achieve deep and precisely normalized profiling of TCR beta repertoires in 39 healthy donors aged 6-90 y. We demonstrate that TCR beta diversity per 10(6) T cells decreases roughly linearly with age, with significant reduction already apparent by age 40. The percentage of naive T cells showed a strong correlation with measured TCR diversity and decreased linearly up to age 70. Remarkably, the oldest group (average age 82 y) was characterized by a higher percentage of naive CD4(+) T cells, lower abundance of expanded clones, and increased TCR diversity compared with the previous age group (average age 62 y), suggesting the influence of age selection and association of these three related parameters with longevity. Interestingly, cross-analysis of individual TCR beta repertoires revealed a set >10,000 of the most representative public TCR beta clonotypes, whose abundance among the top 100,000 clones correlated with TCR diversity and decreased with aging.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available