Journal
JOURNAL OF IMMUNOLOGY
Volume 190, Issue 7, Pages 3639-3647Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1203488
Keywords
-
Categories
Funding
- Superfund Research Program National Institute of Environmental Health Sciences Grant [P42ES013660]
- National Institutes of Health Grant [P20RR018728]
- Environmental Toxicology Training Grant
- Deutsche Forschungsgemeinschaft Grant [BL1115/1-1]
- Grants-in-Aid for Scientific Research [23390386] Funding Source: KAKEN
Ask authors/readers for more resources
Infection and inflammation can disturb immune tolerance at the maternal-fetal interface, resulting in adverse pregnancy outcomes. However, the underlying mechanisms for detrimental immune responses remain ill defined. In this study, we provide evidence for immune programming of fetal loss in response to polyinosinic:polycytidylic acid (polyI:C), a viral mimic and an inducer of inflammatory milieu. IL-10 and uterine NK (uNK) cells expressing the activating receptor NKG2D play a critical role in poly(I:C)-induced fetal demise. In wild type (WT) mice, poly(I:C) treatment induced expansion of NKG2D(+) uNK cells and expression of Rae-1 (an NKG2D ligand) on uterine macrophages and led to fetal resorption. In IL-10(-/-) mice, NKG2D(-) T cells instead became the source of fetal resorption during the same gestation period. Interestingly, both uterine NK and T cells produced TNF-alpha as the key cytotoxic factor contributing to fetal loss. Treatment of WT mice with poly(I:C) resulted in excessive trophoblast migration into the decidua and increased TUNEL-positive signal. IL-10(-/-) mice supplemented with recombinant IL-10 induced fetal loss through NKG2D(+) uNK cells, similar to the response in WT mice. Blockade of NKG2D in poly(I:C)-treated WT mice led to normal pregnancy outcome. Thus, we demonstrate that pregnancy-disrupting inflammatory events mimicked by poly(I:C) are regulated by IL-10 and depend on the effector function of uterine NKG2D(+) NK cells in WT mice and NKG2D(-) T cells in IL-10 null mice. The Journal of Immunology, 2013, 190: 3639-3647.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available