Journal
JOURNAL OF IMMUNOLOGY
Volume 189, Issue 2, Pages 946-955Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1200660
Keywords
-
Categories
Funding
- National Health and Medical Research Council (NHMRC) of Australia
- Operational Infrastructure Support Program
- Victorian Government, Australia
- Cancer Research Institute Scholarship from the Peter MacCallum Cancer Centre/Ludwig Institute for Cancer Research
- Nick Christopher Scholarship from the Department of Medicine, University of Melbourne
- Doherty fellowship from NHMRC
Ask authors/readers for more resources
Although great progress has been made in delineating lung dendritic cell and lymphocyte subpopulations, similar advances in lung macrophages (M Phi s) have been hampered by their intrinsic autofluorescence, cell plasticity, and the complexities of monocyte-M Phi compartmentalization. Using spectral scanning, we define alveolar M Phi autofluorescence characteristics, which has allowed us to develop an alternative flow cytometry method. Using this methodology, we show that mouse lung M Phi s form distinct subpopulations during acute inflammation after challenge with LPS or influenza virus, and in chronic inflammatory lung disease consequent to SHIP-1 deletion. These subpopulations are distinguished by differential Mac-1 and CD11c integrin expression rather than classical M1 or M2 markers, and display differential gene signatures ex vivo. Whereas the resolution of acute inflammation is characterized by restoration to a homogenous population of CD11c(high)Mac-1(neg/low) M Phi s reflective of lung homeostasis, chronic inflammatory lung disease associated with SHIP-1 deficiency is accompanied by an additional subpopulation of CD11c(high)Mac-1(pos) MFs that tracks with lung disease in susceptible genetic background SHIP-(-/-) animals and disease induction in chimeric mice. These findings may help better understand the roles of M Phi subpopulations in lung homeostasis and disease. The Journal of Immunology, 2012, 189: 946-955.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available