Journal
JOURNAL OF IMMUNOLOGY
Volume 189, Issue 5, Pages 2423-2431Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1201302
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Funding
- National Institutes of Health [2 U19 AI062629]
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Gram-positive bacteria are an important public health problem, but it is unclear how they cause systemic inflammation in sepsis. Our previous work showed that peptidoglycan (PGN) induced proinflammatory cytokines in human cells by binding to an unknown extracellular receptor, followed by phagocytosis leading to the generation of NOD ligands. In this study, we used flow cytometry to identify host factors that supported PGN binding to immune cells. PGN binding required plasma, and plasma from all tested healthy donors contained IgG recognizing PGN. Plasma depleted of IgG or of anti-PGN Abs did not support PGN binding or PGN-triggered cytokine production. Adding back intact but not F(ab')(2) IgG restored binding and cytokine production. Transfection of HEK293 cells with Fc gamma RIIA enabled PGN binding and phagocytosis. These data establish a key role for anti-PGN IgG and Fc gamma Rs in supporting inflammation to a major structural element of Gram-positive bacteria and suggest that anti-PGN IgG contributes to human pathology in Gram-positive sepsis. The Journal of Immunology, 2012, 189: 2423-2431.
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