Journal
JOURNAL OF IMMUNOLOGY
Volume 186, Issue 11, Pages 6485-6496Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1001868
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Funding
- National Institutes of Health/National Heart, Lung, and Blood Institute Proteomics Initiative [N01-HV-00245]
- National Institutes of Health/National Center for Research Resources [KL2RR029875]
- National Institute for Environmental Health Science Center [P30-ES006676]
- James W. McLaughlin Predoctoral Fellowship
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The priming of eosinophils by cytokines leading to augmented response to chemoattractants and degranulating stimuli is a characteristic feature of eosinophils in the course of allergic inflammation and asthma. Actin reorganization and integrin activation are implicated in eosinophil priming by GM-CSF, but their molecular mechanism of action is unknown. In this regard, we investigated the role of L-plastin, an eosinophil phosphoprotein that we identified from eosinophil proteome analysis. Phosphoproteomic analysis demonstrated the upregulation of phosphorylated L-plastin after eosinophil stimulation with GM-CSF. Additionally, coimmunoprecipitation studies demonstrated a complex formation of phosphorylated L-plastin with protein kinase C beta II (PKC beta II), GM-CSF receptor alpha-chain, and two actin-associated proteins, paxilin and cofilin. Inhibition of PKC beta II with 4,5-bis (4-fluoroanilino) phtalimide or PKC beta II-specific small interfering RNA blocked GM-CSF-induced phosphorylation of L-plastin. Furthermore, flow cytometric analysis also showed an upregulation of alpha(M)beta(2) integrin, which was sensitive to PKC beta II inhibition. In chemotaxis assay, GM-CSF treatment allowed eosinophils to respond to lower concentrations of eotaxin, which was abrogated by the above-mentioned PKC beta II inhibitors. Similarly, inhibition of PKC beta II blocked GM-CSF induced priming for degranulation as assessed by release of eosinophil cationic protein and eosinophil peroxidase in response to eotaxin. Importantly, eosinophil stimulation with a synthetic L-plastin peptide (residues 2-19) phosphorylated on Ser(5) upregulated alpha(M)beta(2) integrin expression and increased eosinophil migration in response to eotaxin independent of GM-CSF stimulation. Our results establish a causative role for PKC beta II and L-plastin in linking GM-CSF-induced eosinophil priming for chemotaxis and degranulation to signaling events associated with integrin activation via induction of PKC beta II-mediated L-plastin phosphorylation. The Journal of Immunology, 2011, 186: 6485-6496.
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