Journal
JOURNAL OF IMMUNOLOGY
Volume 186, Issue 7, Pages 4443-4454Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1002449
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- National Institutes of Health, National Institute for Dental and Craniofacial Research [DE-13079]
- Michael J. Fox Foundation
- National Institutes of Health/National Institute on Environmental Health Sciences
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The role of the beta 2 adrenergic receptor (beta 2AR) in the regulation of chronic neurodegenerative inflammation within the CNS is poorly understood. The purpose of this study was to determine neuroprotective effects of long-acting beta 2AR agonists such as salmeterol in rodent models of Parkinson's disease. Results showed salmeterol exerted potent neuroprotection against both LPS and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/1-methyl-4-phenylpyridinium-induced dopaminergic neurotoxicity both in primary neuron-glia cultures (at subnanomolar concentrations) and in mice (1-10 mu g/kg/day doses). Further studies demonstrated that salmeterol-mediated neuroprotection is not a direct effect on neurons; instead, it is mediated through the inhibition of LPS-induced microglial activation. Salmeterol significantly inhibited LPS-induced production of microglial proinflammatory neurotoxic mediators, such as TNF-alpha, superoxide, and NO, as well as the inhibition of TAK1-mediated phosphorylation of MAPK and p65 NF-kappa B. The anti-inflammatory effects of salmeterol required beta 2AR expression in microglia but were not mediated through the conventional G protein-coupled receptor/cAMP pathway. Rather, salmeterol failed to induce microglial cAMP production, could not be reversed by either protein kinase A inhibitors or an exchange protein directly activated by cAMP agonist, and was dependent on beta-arrestin2 expression. Taken together, our results demonstrate that administration of extremely low doses of salmeterol exhibit potent neuroprotective effects by inhibiting microglial cell activation through a beta 2AR/beta-arrestin2-dependent but cAMP/protein kinase A-independent pathway. The Journal of Immunology, 2011, 186: 4443-4454.
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