Journal
JOURNAL OF IMMUNOLOGY
Volume 184, Issue 9, Pages 4789-4800Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.0902391
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Funding
- National Institutes of Health [CA96547, CA105001, AI56363]
- Ministry of Education, Science, and Culture of Japan
- Leukemia and Lymphoma Society
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Delineating the relative contributions of B lymphocytes during the course of autoimmune disease has been difficult. Therefore, the effects of depleting all mature B cells using a potent CD20 mAb, or of depleting circulating and marginal zone B cells using a ligand-blocking CD22 mAb, were compared in NZB/W F-1 mice, a model for human systemic lupus erythematosus. Single low-dose mAb treatments depleted B cells efficiently in both NZB/W F-1 and C57BL/6 mice. Prophylactic B cell depletion by repeated CD20 mAb treatments prolonged survival during pristane-accelerated lupus in NZB/W F-1 mice, whereas CD22 mAb had little effect. Despite effective B cell depletion, neither mAb treatment prevented autoantibody generation. In addition, CD20, CD22, and control mAb-treated NZB/W F-1 mice developed anti-mouse IgG autoantibodies in contrast to parental NZB and NZW strains, which may have reduced the effectiveness of B cell depletion. Despite this, low-dose CD20 mAb treatment initiated in 12-28-wk-old mice, and administered every 4 wk thereafter, significantly delayed spontaneous disease in NZB/W F-1 mice. By contrast, B cell depletion initiated in 4-wk-old mice hastened disease onset, which paralleled depletion of the IL-10-producing regulatory B cell subset called B10 cells. B10 cells were phenotypically similar in NZB/W F-1 and C57BL/6 mice, but were expanded significantly in young NZB/W F-1 mice. Thus, B cell depletion had significant effects on NZB/W F-1 mouse survival that were dependent on the timing of treatment initiation. Therefore, distinct B cell populations can have opposing protective and pathogenic roles during lupus progression. The Journal of Immunology, 2010, 184: 4789-4800.
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