Type I IFN Receptor Regulates Neutrophil Functions and Innate Immunity to Leishmania Parasites

Type I IFNs exert diverse effector and regulatory functions in host immunity to viral and nonviral infections; however, the role of endogenous type I IFNs in leishmaniasis is unclear. We found that type I IFNR-deficient (IFNAR(-/-)) mice developed attenuated lesions and reduced Ag-specific immune responses following infection with Leishmania amazonensis parasites. The marked reduction in tissue parasites, even at 3 d in IFNAR(-/-) mice, seemed to be indicative of an enhanced innate immunity. Further mechanistic analyses indicated distinct roles for neutrophils in parasite clearance; IFNAR(-/-) mice displayed a rapid and sustained infiltration of neutrophils, but a limited recruitment of CD11b + Ly-6C + inflammatory monocytes, into inflamed tissues; interactions between IFNAR(-/-), but not wild-type (WT) or STAT1(-/-), neutrophils and macrophages greatly enhanced parasite killing in vitro; and infected IFNAR(-/-) neutrophils efficiently released granular enzymes and had elevated rates of cell apoptosis. Furthermore, although coinjection of parasites with WT neutrophils or adoptive transfer of WT neutrophils into IFNAR(-/-) recipients significantly enhanced infection, the coinjection of parasites with IFNAR(-/-) neutrophils greatly reduced parasite survival in WT recipients. Our findings reveal an important role for type I IFNs in regulating neutrophil/monocyte recruitment, neutrophil turnover, and Leishmania infection and provide new insight into innate immunity to protozoan parasites. The Journal of Immunology, 2010, 184: 7047-7056.