Journal
JOURNAL OF IMMUNOLOGY
Volume 183, Issue 7, Pages 4177-4181Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.0901109
Keywords
-
Categories
Funding
- National Institutes of Health [AI-071085, AI-076989]
- Department of Pathology start-up hinds
Ask authors/readers for more resources
Following influenza virus infection, CD8 T cells encounter mature, Ag-bearing dendritic cells within the draining lymph nodes and undergo activation, programmed proliferation, and differentiation to effector cells before migrating to the lungs to mediate viral clearance. However, it remains unclear whether CD8 T cells continue their proliferation after arriving in the lungs. To address this question, we developed a novel, in vivo, dual-label system using intranasal CFSE and BrdU administration to identify virus-specific CD8 T cells that are actively undergoing cell division while in the lungs. With this technique we demonstrate that a high frequency of virus-specific CD8 T cells incorporate BrdU while in the lungs and that this lung-resident proliferation contributes significantly to the magnitude of the Ag-specific CD8 T cell response following influenza virus infection. The Journal of Immunology, 2009, 183: 4177-4181.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available