Journal
JOURNAL OF IMMUNOLOGY
Volume 182, Issue 4, Pages 2313-2324Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.0803218
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Funding
- Dutch Cancer Foundation [RUL 2005-3259]
- Royal Dutch Academy of Sciences Beijerinck Premium 2005
- Netherlands Scientific Organization (NWO) Vidi [917.76.330]
- MRC [G0501074, G9901249, G9818340] Funding Source: UKRI
- Medical Research Council [G9818340, G0501074, G9901249] Funding Source: researchfish
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EBV persists for life in the human host while facing vigorous antiviral responses that are induced upon primary infection. This persistence supports the idea that herpesviruses have acquired dedicated functions to avoid immune elimination. The recently identified EBV gene product BNLF2a blocks TAP. As a result, reduced amounts of peptides are transported by TAP from the cytoplasm into the endoplasmic reticulum (ER) lumen for binding to newly synthesized HLA class I molecules. Thus, BNLF2a perturbs detection by cytotoxic T cells. The 60-aa-long BNLF2a protein prevents the binding of both peptides and ATP to TAP, yet further mechanistic insight is, to date, lacking. In this study, we report that EBV BNLF2a represents a membrane-associated protein that colocalizes with its target TAP in subcellular compartments, primarily the ER. In cells devoid of TAP, expression levels of BNLF2a protein are greatly diminished, while ER localization of the remaining BNLF2a is retained. For interactions of BNLF2a with the HLA class I peptide-loading complex, the presence of TAP2 is essential, whereas tapasin is dispensible. Importantly, we now show that in B cells supporting EBV lytic replication, the BNLF2a protein is expressed early in infection, colocalizing and associating with the peptide-loading complex. These results imply that, during productive EBV infection, BNLF2a contributes to TAP inhibition and surface HLA class I down-regulation. In this way, EBV BNLF2a-mediated evasion from HLA class I-restricted T cell immunity contributes to creating a window for undetected virus production. The Journal of Immunology, 2009, 182: 2313-2324.
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