Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 405, Issue -, Pages 144-153Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2014.02.003
Keywords
Alveolar macrophage; Cytokines; Inflammation; Chronic obstructive pulmonary disease; Corticosteroids; p38 MAPK
Categories
Funding
- GlaxoSmithKline
- BBSRC
- Medical Research Council [MR/L00254X/1, MR/L010240/1] Funding Source: researchfish
- MRC [MR/L00254X/1, MR/L010240/1] Funding Source: UKRI
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It is unclear whether cell culture methodology affects the corticosteroid sensitivity of chronic obstructive pulmonary disease (COPD) alveolar macrophages. We compared the effect of a short and a long isolation procedure on corticosteroid inhibition of lipopolysaccharide (LPS) stimulated cytokine release from COPD alveolar macrophages. We also investigated signalling pathways associated with macrophage activation during cell isolation. Macrophages cultured using a short isolation protocol released higher unstimulated levels of tumour necrosis factor (TNF)-alpha and chemokine C-X-C motif ligand (CXCL) 8; these macrophages were less sensitive to corticosteroid inhibition of LPS stimulated TNF-alpha and CXCL8 release when compared to a long isolation procedure. This was associated with increased p38 mitogen activated kinase (MAPK) activation. The p38 MAPK inhibitor, BIRB-796, significantly reduced unstimulated cytokine release. A key finding of this study was that both cell culture methods showed no difference in the corticosteroid sensitivity between COPD and control macrophages. We conclude that the culture of alveolar macrophages using a short isolation procedure alters cytoldne production through p38 MAPK activation; this is associated with a change in corticosteroid sensitivity. (C) 2014 The Authors. Published by Elsevier B.V.
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