Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 408, Issue -, Pages 89-100Publisher
ELSEVIER
DOI: 10.1016/j.jim.2014.05.009
Keywords
Cre-recombinase; Floxed; Dendritic cell; Macrophage; Neutrophil
Categories
Funding
- US National Institutes of Health [AI065495, AI068150, AI078869]
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Since the first example of conditional gene targeting in mice in 1994, the use of Cre recombinase and loxP flanked sequences has become an invaluable technique to generate tissue and temporal specific gene knockouts. The number of mouse strains expressing foxed-gene sequences, and tissue-specific or temporal-specific Cre-recombinase that have been reported in the literature has grown exponentially. However, increased use of this technology has highlighted several problems that can impact the interpretation of any phenotype observed in these mouse models. In particular, accurate knowledge of the specificity of Cre expression in each strain is critical in order to make conclusions about the role of specific cell types in the phenotypes observed. Cre-mediated deletion specificity and efficiency have been described in many different ways in the literature, making direct comparisons between these Cre strains impossible. Here we report crossing thirteen different myeloid-Cre mouse strains to ROSA-EYFP reporter mice and assaying YFP expression in a variety of naive unstimulated hematopoietic cells, in parallel. By focusing on myeloid subsets, we directly compare the relative efficiency and specificity of myeloid deletion in these strains under steady-state conditions. (C) 2014 Elsevier B.V. All rights reserved.
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