Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 408, Issue -, Pages 24-34Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2014.04.010
Keywords
CD16a; CD64; Monoclonal antibody; Glycosylation; Surface plasmon resonance (SPR); Anti-histidine capture; Aggregation
Categories
Funding
- NSERC Strategic Network for The Production of Single-type Glycoform Monoclonal Antibodies (MabNet) group
Ask authors/readers for more resources
We here report the production and purification of the extracellular domains of two Fc gamma receptors, namely CD16a and CD64, by transient transfection in mammalian cells. The use of these two receptor ectodomains for the development of quantitative assays aiming at controlling the quality of monoclonal antibody production lots is then discussed. More specifically, the development of surface plasmon resonance-based biosensor assays for the evaluation of the glycosylation pattern and the aggregation state of monoclonal antibodies is presented. Our biosensor approach allows discriminating between antibodies harboring different galactosylation profiles as well as to detect low levels (i.e., less than 2%) of monoclonal antibody aggregates. (C) 2014 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available