Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 365, Issue 1-2, Pages 149-157Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2010.12.012
Keywords
Rabbit monoclonal antibody; Embryonic stem cells; Surface antigens
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Funding
- National Key Scientific Research Program of China [2007CB947804]
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Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of the blastocyst. ES cell surface molecules are important for the identification, labeling, sorting, quality control and functional studies of ES cells. Currently, knowledge of ES surface molecules is limited. To identify new surface molecules, we generated a panel of rabbit monoclonal antibodies (rMabs) against mouse ES (mES) cells. We identified three monoclonal antibodies that interact with molecules on the mES cell surface and found that the expression of their respective antigens decreased upon mES cell differentiation. The antigen of the rMab ZjuESrMab29 was identified as granulocyte macrophage colony-stimulating factor receptor (GM-CSFR alpha) by liquid chromatography-tandem mass spectrometry (LC-MS/MS). This study demonstrated that rabbit monoclonal antibody production via whole-cell immunization could be a practical method for the discovery of stem cell surface antigens. (C) 2010 Elsevier B.V. All rights reserved.
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