Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 347, Issue 1-2, Pages 3-11Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2009.06.003
Keywords
Immunoassays; Tandem mass spectrometry; Autoantibody interference; Hook effect; Heterophile anti-reagent antibodies; Standardization
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Funding
- NIDDK NIH HHS [P30 DK035816-229003, P30 DK035816] Funding Source: Medline
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Immunoassays have made it possible to measure dozens of individual proteins and other analytes in human samples for help in establishing the diagnosis and prognosis of disease. In too many cases the results of those measurements are misleading and can lead to unnecessary treatment or missed opportunities for therapeutic interventions. These cases stem from problems inherent to immunoassays performed with human samples, which include a lack of concordance across platforms, autoantibodies, anti-reagent antibodies, and the high-dose hook effect. Tandem mass spectrometry may represent a detection method capable of alleviating many of the flaws inherent to immunoassays. We review our understanding of the problems associated with immunoassays on human specimens and describe methodologies using tandem mass spectrometry that could solve some of those problems. We also provide a critical discussion of the potential pitfalls of novel mass spectrometric approaches in the clinical laboratory. (C) 2009 Elsevier B.V. All rights reserved.
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