Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 346, Issue 1-2, Pages 55-63Publisher
ELSEVIER
DOI: 10.1016/j.jim.2009.05.004
Keywords
CD39; Ectonucleotidase; Regulatory T cells; FOXP3; CD4(+)CD25(high)
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Funding
- Philip Morris International
- [PO-I CA109688]
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Human regulatory T cells (Treg) have been variously defined as CD4(+)CD25(+), CD4(+)CD25(high) or CD4(+)CD25(high)FOXP(3+) cells which are responsible for maintaining peripheral tolerance. Their isolation from human peripheral blood or tissues depends on the expression level of CD25(IL-2R alpha) - a surface marker which is also expressed on activated effector helper T cells. CD39, a cell surface associated ectonucleotidase, can be used to purify Treg with strong suppressor functions. The CD4(+)CD39(+) T cells catalyze cleavage of adenosine triphosphate (ATP) to adenosine monophosphate (AMP), which is then further cleaved to adenosine. CD4(+)CD39(+) T cells largely overlap with CD4(+)CD25(high)FOXP(3+) but not CD4(+)CD25(+) T cell subset, and mediate equally potent immune suppression. Thus, CD39 surface marker can be successfully used for routine isolation of functionally-active human Treg from the peripheral blood of healthy donors or patients with cancer for studies of their role in health and disease. (c) 2009 Elsevier B.V. All rights reserved.
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