Journal
ORGANIC LETTERS
Volume 17, Issue 21, Pages 5344-5347Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.orglett.5b02707
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Funding
- Pew Scholars Program
- National Institutes of Health [DP2AT009148]
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The cyclizidine biosynthetic gene cluster was identified from Streptomyces NCIB 11649, which revealed the polyketide biosynthetic machinery for cyclizidine alkaloid biosynthesis. Both in vivo mutagenesis study and in vitro biochemical analysis provided insight into the timing and mechanism of the biosynthetic enzymes that produce cyclizidine-type indolizidine alkaloids.
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