Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 350, Issue 1-2, Pages 1-13Publisher
ELSEVIER
DOI: 10.1016/j.jim.2009.07.006
Keywords
Sulfonamide; ELISA; Edible animal products; Broad specificity antibody
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Funding
- Ministry of Science and Technology of the People's Republic of China [2006AA10Z448]
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The current status of enzyme-linked immunosorbent assays (ELISAs) for sulfonamides in edible animal products is reviewed. The attention was focused on the design and synthesis of haptens, conjugation to carrier protein, production of antibody, application of homologous and heterologous systems, as well as the molecular modeling of the haptens and sulfonamides. Researches have shown that sulfonamides seem to be particularly resistant to attempts to produce broad specificity antibodies. By summarizing the available research on sulfonamide ELISAs, it is hoped that it can be considered as a basis for further investigation aimed at developing the most efficient approaches for detection. (C) 2009 Elsevier B.V. All rights reserved.
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