Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 340, Issue 1, Pages 33-41Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2008.09.021
Keywords
Malaria; MIG; CXCL9; Vaccine; IFN-gamma; Flow cytometry
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Funding
- Medical Research Council [G0500634, G84/6323] Funding Source: Medline
- Medical Research Council [G0500634, G84/6323] Funding Source: researchfish
- MRC [G0500634, G84/6323] Funding Source: UKRI
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For many years the IFN-gamma ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced by IFN-gamma and has the potential to provide amplification of the IFN-gamma signal. MIG secretion could provide a measure of bio-active IFN-gamma and a functional IFN-gamma signalling pathway. We report that detecting MIG by flow cytometry and by RT-PCR can be more sensitive than the detection of IFN-gamma using these methods. We also find that there is little inter-individual variability in MIG secretion when detected by flow cytometry and that the MIG assay may be used to estimate the amount of bio-active IFN-gamma present. Measurement of MIG alongside IFN-gamma may provide a fuller picture of Th1 type responses post-vaccination. (c) 2008 Elsevier B.V. All rights reserved.
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