Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 332, Issue 1-2, Pages 170-174Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2007.11.012
Keywords
mouse; lymph node; lymphatic drainage; Evans Blue; lymphography
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Funding
- NCI NIH HHS [R01 CA068328-11, R01 CA068328, R01 CA 68328] Funding Source: Medline
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Accurate identification of lymph nodes in the mouse is critical for studies of tumor metastasis, and of regional immune responses following immunization. However, these small lymphatic organs are often difficult to identify in mice using standard dissection techniques, so that larger rats have been used to characterize rodent lymphatic drainage. We developed techniques injecting dye into the mouse footpad or tail, to label the lymphatic drainage of the hind leg and flank, pelvic viscera, prostate and mammary glands. While lymphatic drainage patterns were similar in mice and rats, the inguinal lymph nodes showed distinct differences in afferent and efferent drainage. These techniques allow accurate and rapid identification of lymph nodes and lymphatic drainage in normal as well as diseased mice. Published by Elsevier B.V.
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