Journal
JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY
Volume 31, Issue 4, Pages 266-278Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15321819.2010.524857
Keywords
dehydroepiandrosterone; heterologous; homologous; immunoassay; site heterology
Funding
- National Institute of Health and Family Welfare, New Delhi, India
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Homologous and heterologous combinations of enzyme conjugate with immunogen in steroid enzyme immunoassay (EIA) influence labeled steroid recognition by an antibody that affects the sensitivity of the assay. To develop dehydroepiandrosterone (DHEA) enzyme linked immunosorbent assay (ELISA), antibodies were generated against dehydroepiandrosterone-17-carboxymethyloxime-bovine serum albumin (DHEA-17-CMO-BSA) and dehydroepiandrosterone-7-carboxymethyloxime- bovine serum albumin (DHEA-7-CMO-BSA). Two dehydroepiandrosterone (DHEA) horse radish peroxidise (HRP) enzyme conjugates were prepared using two dehydroepiandrosterone derivatives (DHEA-17-CMO and DHEA-7-CMO). Four combinations of homologous and heterologous assays were evaluated. The use of heterologous combination improved the sensitivity of the assay.
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