Biodegradation of 25-norhopanes in a Liaohe Basin (NE China) oil reservoir

A suite of reservoir cores in an oil column from the Shuguang oil field, Liaohe Basin, NE China was characterized geochemically to investigate the behaviour of 25-norhopanes (NHs) during biodegradation. The extracted bitumens show similar organofacies type and maturity levels with biodegradation as the primary control on compositional variation. All bitumens suffered severe biodegradation influence which removed all regular steranes and most of the pentacyclic terpanes leaving NHs as dominant components in total ion chromatograms of the saturated hydrocarbon fraction. However, the NHs are not the endpoint of hopane destruction: they are biodegraded as well. Biodegradation of NHs can be illustrated by significant depletion in their concentrations and systematic variation in ratios of resistant to vulnerable components throughout the oil column. The biodegradation sequence is somewhat similar to normal pentacyclic terpanes. Among the NH series, 18 alpha-25,30-bisnorneohopane is the most recalcitrant to biodegradation. The 18 alpha-22,25,29,30-tetranorneohopane is more resistant to biodegradation than 17 alpha-22,25,29,30- tetranorhopane and 34NHs are more resistant than other NHs. Degradation of NHs provides a new angle to understanding the complicated inter-relationships in subsurface biodegraded reservoirs. (C) 2014 Elsevier Ltd. All rights reserved.