Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 13, Issue 4, Pages 1068-1081Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ob02087a
Keywords
-
Categories
Funding
- University of the Basque Country UPV/EHU [UFI 11/32]
- National Council of Science and Technology (CONAYT), Mexico [217101]
- Spanish Ministry of Education [CTQ2010-20541, CTQ2010-14897]
- Basque Government (Department of Education, University and Research) [BFI-20112226]
- Generalitat de Catalunya [2009SGR208, 2009SGR1331]
- Instituto de Salud Carlos III
Ask authors/readers for more resources
We designed niosomes based on three lipids that differed only in the polar-head group to analyze their influence on the transfection efficiency. These lipids were characterized by small-angle X-ray scattering before being incorporated into the niosomes which were characterized in terms of pK(a), size, zeta potential, morphology and physical stability. Nioplexes were obtained upon the addition of a plasmid. Different ratios (w/w) were selected to analyze the influence of this parameter on size, charge and the ability to condense, release and protect the DNA. In vitro transfection experiments were performed in HEK-293, ARPE-19 and MSC-D1 cells. Our results show that the chemical composition of the cationic head-group clearly affects the physicochemical parameters of the niosomes and especially the transfection efficiency. Only niosomes based on cationic lipids with a dimethyl amino head group (lipid 3) showed a transfection capacity when compared with their counterparts amino (lipid 1) and tripeptide head-groups (lipid 2). Regarding cell viability, we clearly observed that nioplexes based on the cationic lipid 3 had a more deleterious effect than their counterparts, especially in ARPE-19 cells at 20/1 and 30/1 ratios. Similar studies could be extended to other series of cationic lipids in order to progress in the research on safe and efficient non-viral vectors for gene delivery purposes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available