Journal
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES
Volume 34, Issue 1, Pages 33-36Publisher
SPRINGER
DOI: 10.1007/s11596-014-1228-x
Keywords
aortic valve calcification; valve interstitial cells; Wnt/beta-catenin signaling pathway; glycogen synthase kinase 3 beta; beta-catenin
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Funding
- National Natural Science Foundation of China [81070190]
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Aortic valve calcification is a common disease in the elderly, but its cellular and molecular mechanisms are not clear. In order to verify the hypothesis that Wnt/beta-catenin signaling pathway is involved in the process of calcification of aortic valve, porcine aortic valve interstitial cells (VICs) were isolated, cultured and stimulated with oxidized low density lipoprotein (ox-LDL) for 48 h to induce the differentiation of VICs into osteoblast-like cells. The key proteins and genes of Wnt/beta-catenin signaling pathway, such as glycogen synthase kinase 3 beta (GSK-3 beta) and beta-catenin, were detected by using Western blotting and real-time polymerase chain reaction (PCR). The results showed that the VICs managed to differentiate into osteoblast-like cells after the stimulation with ox-LDL and the levels of proteins and genes of GSK-3 beta and beta-catenin were increased significantly in VICs after stimulation for 48 h (P < 0.05). It is suggested that Wnt/beta-catenin signaling pathway may play a key role in the differentiation of VICs into osteoblast-like cells and make great contribution to aortic valve calcification.
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