Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 60, Issue 10, Pages 723-733Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1369/0022155412453052
Keywords
imaging flow cytometry; flow cytometry; biological heterogeneity; fluorescence; cellular morphology; Poisson distribution; single cell; high-throughput fluorescent microscopy
Categories
Funding
- NIH [S10 RR023459]
- Immune Disease Institute
- RFBR [11-04-01749a]
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Imaging flow cytometry (IFC) platforms combine features of flow cytometry and fluorescent microscopy with advances in data-processing algorithms. IFC allows multiparametric fluorescent and morphological analysis of thousands of cellular events and has the unique capability of identifying collected events by their real images. IFC allows the analysis of heterogeneous cell populations, where one of the cellular components has low expression (<0.03%) and can be described by Poisson distribution. With the help of IFC, one can address a critical question of statistical analysis of subcellular distribution of proteins in a cell. Here the authors review advantages of IFC in comparison with more traditional technologies, such as Western blotting and flow cytometry (FC), as well as new high-throughput fluorescent microscopy (HTFM), and discuss further developments of this novel analytical technique. (J Histochem Cytochem 60:723-733, 2012)
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